Identifying antisense oligonucleotide binding
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United States of America Patent
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Dec 31, 2002
Grant Date -
N/A
app pub date -
May 26, 1999
filing date -
Oct 4, 1996
priority date (Note) -
Expired
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Abstract
A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, which comprises contacting the target mRNA with each member of an oligonucleotide library separately under hybridization conditions, removing unhybridized material and determining which member or members hybridize; wherein the oligonucleotide library comprises a plurality of distinct nucleotide sequences of a predetermined common length, and wherein each nucleotide sequence comprises a known sequence of 4 to 8 bases and all possible combinations of the known sequence are present in the library.
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