Particle transfection: rapid and efficient transfer of polynucleotide molecules into cells
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United States of America Patent
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Jan 7, 2003
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N/A
app pub date -
Oct 13, 1992
filing date -
Oct 13, 1992
priority date (Note) -
Expired
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Abstract
A rapid, simple method of directly transfecting a large number of eukaryotic, prokaryotic or plant cells, which retains substantial cell viability is achieved by the present invention. The method includes the steps of contacting with cells adhered to a support, an amount of polynucleotide molecule targeted for transfection into the cells and an amount of particles. A gentle agitation of the cells, polynucleotide molecules and particles permits direct transfection of the polynucleotide molecules into the cells.
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