Analytical element and method for the determination of an analyte in a liquid

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United States of America Patent

PATENT NO 6506575
SERIAL NO

09667931

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Abstract

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The invention concerns an analytical element for determining the amount of an analyte in a liquid containing a sample application zone (1) and a detection zone (2) which are in liquid-transferring contact, the latter containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and containing a liquid-permeable interference-reducing layer (3) in direct contact with the detection zone (2) arranged such that liquid does not reach the detection zone until it has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance, and a method for the determination of an analyte in a liquid by means of such an analytical element.

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Patent Owner(s)

Patent OwnerAddress
ROCHE DIAGNOSTICS GMBH68305 MANNHEIM

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gaa, Otto Worms, DE 16 401
Knappe, Wolfgang Reinhold Ludwigshafen, DE 6 204
Pachl, Rudolf Ellerstadt, DE 20 1145

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