DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
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United States of America Patent
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Jan 14, 2003
Grant Date -
Sep 13, 2001
app pub date -
Mar 26, 1999
filing date -
Mar 31, 1998
priority date (Note) -
Expired
status (Latency Note)
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Abstract
This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, the sequences being linked in order, the signal peptide, tag and linker being optional sequences; and wherein the nucleotide sequence encoding a fusion protein is ligated to 3'-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.
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