Purification of alpha-1 proteinase inhibitor
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United States of America Patent
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Feb 25, 2003
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N/A
app pub date -
Jul 7, 2000
filing date -
Sep 7, 1995
priority date (Note) -
Expired
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Abstract
A method for purifying human or other alpha-l proteinase inhibitor (.alpha..sub.1 -PI) from a solution (which may be derived from the milk of a transgenic animal expressing the .alpha..sub.1 -PI) which comprises contacting the solution with a cation exchange substrate under conditions sufficient to bind non-tg-.alpha..sub.1 -PI contaminants to the substrate while not substantially binding tg .alpha..sub.1 -PI to the substrate. Using the preferred embodiment, the purified tg .alpha..sub.1 -PI contains as little as 40 pg non-.alpha..sub.1 -PI-whey protein per mg total protein.
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