Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA

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United States of America Patent

PATENT NO 6528256
SERIAL NO

09603613

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Abstract

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The present invention is directed to a method for the identification and isolation of specific genetic sequences from cDNA libraries or samples of genomic DNA derived from the cells, tissues or organs of an organism, particularly a mammal, and most particularly a human (including a human embryo or fetus). In particular, the invention is related to a method whereby a tissue-specific cDNA or a genetic marker, not found in cDNA libraries or genomic DNA samples derived from other tissues from the same or a different organism, may be identified and isolated using an Amplification Fragment Length Polymorphism (AFLP)-based technique. This method has applications in the identification and isolation of cDNA sequences and genomic genetic markers which may be used in a variety of medical, forensic, industrial and plant breeding procedures.

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Patent Owner(s)

  • INVITROGEN CORPORATION

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kuo, Jonathan Germantown, MD 6 26
Li, Wu-Bo Gaithersburg, MD 24 398
Lin, Jhy-Jhu Potomac, MD 42 560

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