Two-step hybridization and capture of a polynucleotide
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United States of America Patent
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Mar 18, 2003
Grant Date -
Sep 12, 2002
app pub date -
Sep 18, 2001
filing date -
May 2, 1997
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Abstract
A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions that control the order of hybridization, where the first hybridization condition allows hybridization of the capture probe to the target polynucleotide, and the second hybridization condition allows hybridization of the capture probe to the immobilized probe. The method further includes amplifying the captured target polynucleotide by hybridizing at least one primer oligonucleotide to the target polynucleotide and using nucleic acid amplification that initiates from the primer oligonucleotide.
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