Methods for enhancing binding interactions between members of specific binding pairs
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United States of America Patent
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Apr 22, 2003
Grant Date -
N/A
app pub date -
Jan 29, 1999
filing date -
Jan 29, 1999
priority date (Note) -
Expired
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Abstract
Disclosed is a method that improves the efficiency of reactions between specific binding pairs. By translating one member of the pair through a suspension of the second member, or across a surface to which the second member is immobilized, collision and binding frequencies are greatly increased. The resulting increased binding frequency allows for reduced incubation times and lower incubation temperatures. The enhanced collisions are advantageous for both molecular reactions, in which mixing is not always effective, and cellular reactions, in which the cells may be damaged by mixing.
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