Detection of altered expression of genes regulating cell proliferation
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United States of America Patent
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May 13, 2003
Grant Date -
N/A
app pub date -
Oct 15, 1999
filing date -
Oct 15, 1998
priority date (Note) -
Expired
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Abstract
The present invention relates to fluorescent probes which can be used in multicolor fluorescence in situ hybridization, and mainly chromosome painting. The probes intended for labeling a chromosome are such that they are composed of a set of DNA segments which are more represented in certain chromosome bands and which are obtained by IRS-PCR amplification from said chromosomes using PCR primers specific for the repeated and dispersed Alu and LINE DNA sequences. The invention comprises, in addition, methods of producing said probes, multicolor FISH methods which can use said probes as well as diagnostic kits comprising them. Finally, the invention comprises combinations of fluorophores and optical filters.
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