Method and composition for the diagnosis of equine infectious anemia virus disease by using the recombinant capsid protein virus (p26)
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United States of America Patent
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Jul 22, 2003
Grant Date -
Mar 7, 2002
app pub date -
Jan 16, 2001
filing date -
Dec 18, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to a method and kit for detecting antibodies in clinical samples of animals infected with equine infectious anemia virus using the immunodiagnosis with the recombinant viral antigen p26. The antigen was bound to solid supports (microtitter plates, tubes, beads or nitrocellulose papers or nylon) and reacted with the test serum. After incubation with conjugated anti-equine immunoglobulin-enzyme the reaction was revealed with a solution composed of the substrate of the enzyme used in the conjugate (cromogene). After development of the reaction (color formation) it was stopped with acid solution and measured. The immunoassay may be a direct second antibody immunoassay, a one or two step sandwich immunoassay.
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