Methods and arrays for detecting biomolecules

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United States of America Patent

PATENT NO 6602661
SERIAL NO

09718990

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Abstract

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The present invention is directed to a device and a method for detecting biomolecules in a tissue section or other two-dimensional sample by creating 'carbon copies' of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using antibodies or DNA probes having specific affinity for the biomolecules of interest. Thin membranes in a stacked or layered configuration are applied to the sample, such as a tissue section, and reagents and reaction conditions are provided so that the biomolecules are eluted from the sample and transferred onto each of the stacked membranes thereby producing multiple replicas of the biomolecular content of the sample.

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Patent Owner(s)

Patent OwnerAddress
GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES THEBETHESDA MD 20892 UNITED STATES OF AMERICA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Emmert-Buck, Michael R Silver Spring, MD 25 309
Knezevic, Vladimir Silver Spring, MD 10 116

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