Methods and devices for multiplexing amplification reactions
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United States of America Patent
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Aug 12, 2003
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N/A
app pub date -
Jun 6, 2000
filing date -
Jun 6, 2000
priority date (Note) -
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Abstract
The present invention pertains to methods, reagents, compositions, kits, and instruments for use in simultaneously amplifying multiple targets. In particular, this invention is based on the discovery of a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to 'boost' the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid.
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