System and method of fluorescence spectroscopic imaging for characterization and monitoring of tissue damage
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United States of America Patent
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Oct 7, 2003
Grant Date -
Jan 3, 2002
app pub date -
Jan 22, 2001
filing date -
Jan 20, 2000
priority date (Note) -
Expired
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Abstract
A method for monitoring a biological tissue includes illuminating the tissue, including a fluorophore, with a wavelength of light, the wavelength selected for exciting the fluorophore, determining a fluorescent emission of the fluorophore, the emission indicating the presence of the fluorophore, and correlating an emission of the fluorophore to an extent and degree of damage to the tissue. Damage to the tissue includes a breakdown of the fluorophore, resulting in a reduced level of emission. The fluorophore can include one of collagen and elastin. The fluorophore can include tryptophan, nicotinamide adenine dinucleotide, flavin and porphyrin. Correlating the emission of the fluorophore to the extent and degree of damage further includes processing a correlation of the emission over time, controlling the power of a laser welder based on the processed correlation, and preventing overheating of the tissue by the laser welder.
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