Method for sequencing reaction cleanup by constant differential pressure ultrafiltration
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United States of America Patent
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Nov 11, 2003
Grant Date -
Aug 1, 2002
app pub date -
Oct 19, 2001
filing date -
Sep 17, 1999
priority date (Note) -
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Abstract
A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of: providing a defined quantity of sequencing reaction product; providing at least one ultrafiltration membrane having at least one surface; transferring the suspended sequencing reaction product to the surface of the ultrafiltration membrane; and applying a first constant pressure differential to the ultrafiltration membrane at a force capable of producing the sequencing reaction product substantially free of the contaminants.
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