Methods and reagents for the rapid and efficient isolation of circulating cancer cells
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United States of America Patent
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Nov 11, 2003
Grant Date -
Jan 24, 2002
app pub date -
Jul 13, 2001
filing date -
Feb 12, 1998
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Abstract
A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.
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