Celiac antigen
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United States of America Patent
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Nov 25, 2003
Issued Date -
N/A
app pub date -
Mar 5, 1999
filing date -
Mar 29, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
In this invention a novel antigen and a novel antibody-antigen complex are provided for screening patients suspected of having celiac disease. The antigen is prepared by starting with human placenta tissue which is perfused with a hepes and collagenase buffer to obtain a single cell suspension. This suspension is enriched to obtain an enriched protein portion and then separated out to obtain an embryonic celiac antigen (ECA). This ECA is used with serum from patients to effect binding of ECA with IgA in the serum while applying a human IgA antibody to the serum. The results are then read on a spectrophotometer to confirm or negate the presence of celiac disease.
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