Method of measuring total homocysteine
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United States of America Patent
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Feb 3, 2004
Grant Date -
Sep 5, 2002
app pub date -
Feb 28, 2002
filing date -
Jun 30, 2000
priority date (Note) -
Expired
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Abstract
The present invention provides a method and a kit for detecting or quantitatively determining homocysteine rapidly and simply and with high sensitivity by oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and determining the produced hydrogen peroxide by color development using an oxidative color-developing agent. By using the method and kit of the present invention, homocysteine in biological samples, in particular, in body fluids such as blood and urine can be detected and quantitatively determined rapidly and simply and with high sensitivity.
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