Processing fetal or neo-natal tissue to produce a scaffold for tissue engineering
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United States of America Patent
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Feb 24, 2004
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app pub date -
May 31, 2001
filing date -
Dec 4, 2000
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Abstract
A method of forming and preserving a bioremodelable, biopolymer scaffold material is carried out by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The process may include, but is not limited to, harvesting the tissue, optionally extracting growth and differentiation factors from the tissue, inactivating infective agents of the tissue such as by treating with KOH or NaOH, mechanically expressing undesirable components from the tissue, delipidizing the tissue with organic solvents, mechanically expressing components released by the solvet, washing the tissue, optionally drying the tissue, and optionally cross-linking the tissue, not necessarily in the order described. The resulting product, named EB Matrix (EBM), is characterized by its microbial, fungal, viral and prion inactivated state. EBM is strong, bioremodelable, drapable and does not undergo calcification.
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