Enzyme substrate delivery and product registration in one step enzyme immunoassays
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United States of America Patent
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Mar 16, 2004
Grant Date -
Feb 28, 2002
app pub date -
Aug 29, 2001
filing date -
Nov 24, 1997
priority date (Note) -
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Abstract
One-step enzyme immunoassays in which enzyme-antibody conjugate or label and enzyme substrate are separated until separation of bound and free enzyme conjugate or label is complete. This separation is accomplished by using variable flow paths, immobilization of substrate at the test line, placement of substrate in a sac or association with a particle label, enzyme product chemical capture, delay zone dissolution and protected enzyme substrates.
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