Methods for identifying species or Shigella and E. coli using operon sequence analysis
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United States of America Patent
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Apr 27, 2004
Grant Date -
May 8, 2003
app pub date -
Feb 2, 1998
filing date -
Feb 20, 1997
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Expired
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Abstract
A method for comparing the variable reactivity of multiple, differentially mutated copies of 16S subsequences found in a number of ribosomal RNA operons of a single bacterial cell is described. The application of this method for distinguishing between closely related organisms, such as the genera Escherichia and Shigella, and between species of Shigella including S. boydii, S. dysenteriae, S. flexneri, and S. sonnei using nucleic acid probes is also presented.
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