Method, vessel and device for monitoring metabolic activity of cell cultures in liquid media
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United States of America Patent
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May 4, 2004
Grant Date -
N/A
app pub date -
Jul 27, 2001
filing date -
Jan 29, 1999
priority date (Note) -
Expired
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Abstract
The invention relates to a method for monitoring metabolic activity of cells in liquid media, to an appropriate vessel and to a device for implementing said method. The invention aimes at enhancing measuring accuracy and reducing the costs involved in monitoring oxygen consumption and thus metabolic activity in culture cells. To this end, oxygen concentration is optically determined with the aid of sensor membranes in a liquid medium located in partially oxygen permeable vessels. Then, the measured oxygen concentrations in the vessels containing culture cells in a liquid medium are compared with the measured oxygen concentration in a vessel containing only such a liquid medium without cells and/or with an oxygen concentration calculated using the measured values of other parameters.
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