Importance

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Abstract

A mesh is created from selected focus points having locations on a surface of interest of a sample being micro-imaged. The mesh and associated focus settings of the focus points are used to define adjacent focus facets forming a focus surface substantially coincident with the surface of interest of the sample. In micro-imaging the sample, a micro-imaged portion of the surface of interest is segmented for the purpose of acquiring tile images. A tile image focus location is used to extract a tile image focus setting from the focus surface. A tile image focus determination process selects a focus facet coincident with the tile image focus location and interpolates a tile image focus setting from focus settings associated with the focus points defining the focus facet. If a coincident focus facet is not found, the tile image focus setting is set to the focus setting of a nearest focus point. Dependence on autofocus is thus eliminated, providing faster imaging and better focused images.

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First Claim