Method for rapid thermal cycling of biological samples
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United States of America Patent
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Sep 7, 2004
Grant Date -
Jul 12, 2001
app pub date -
Aug 11, 1998
filing date -
Jun 4, 1990
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achieved by the present invention.
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