Mobile phase dilution scheme for enhanced chromatography
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United States of America Patent
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Sep 14, 2004
Grant Date -
Feb 26, 2004
app pub date -
Apr 14, 2003
filing date -
Nov 21, 2000
priority date (Note) -
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Abstract
A method and device for increasing the loading capacity of a chromatography column through dilution of a mobile phase at the head of the column. A strong mobile phase is provided for dissolving a sample. A sample is injected into the strong mobile phase, and subsequently diluted with a weak mobile phase. The resulting sample-containing weak mobile phase is passed through a chromatography column for retention and separation of the components of the sample.
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