Method of purifying lantibiotics
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United States of America Patent
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Sep 21, 2004
Grant Date -
Apr 15, 2004
app pub date -
Oct 9, 2002
filing date -
Oct 9, 2002
priority date (Note) -
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status (Latency Note)
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Abstract
Disclosed is a method for purifying a antibiotic from a crude or partially purified solution containing the lantibiotic. In preferred embodiments, the lantibiotic is nisin, although the common structural features of lantibiotics dictate the effectiveness of the disclosed purification methods for other members of the lantibiotic genus. The method includes the step of forming an incubation mixture comprising the solution containing the lantibiotic and a proteolytic enzyme, and incubating the mixture under conditions optimized for selective proteolytic activity.
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