Method and apparatus for MALDI analysis
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United States of America Patent
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Nov 2, 2004
Issued Date -
N/A
app pub date -
Apr 3, 2002
filing date -
Sep 19, 1996
priority date (Note) -
Expired
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Abstract
Matrix assisted laser desorption/ionization (MALDI) is performed in a manner that thermalizes large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus 'thermalized' the analyte ions are passed to a mass analysis instrument.
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