Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
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United States of America Patent
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Nov 9, 2004
Grant Date -
Apr 24, 2003
app pub date -
Oct 24, 2001
filing date -
Jan 23, 1996
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Abstract
Methods are provided for detecting the binding of a first member to a second member of a ligand pair, comprising the steps of (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry, or potentiometry, (b) separating bound first and second members from unbound members, (c) cleaving the tag from the tagged first member, and (d) detecting the tag by non-fluorescent spectrometry, or potentiometry, and therefrom detecting the binding of the first member to the second member.
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