Method and apparatus for determining gene expression levels
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United States of America Patent
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Nov 9, 2004
Grant Date -
Jul 1, 2004
app pub date -
Feb 13, 2003
filing date -
Dec 31, 2002
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Techniques for analyzing gene expression levels are provided. In one aspect of the invention, the technique provides a method for determining a concentration level of a target nucleic acid, the target nucleic acid comprising at least one target oligonucleotide. The method determines (i) a measure of affinity value of the target oligonucleotide with a probe oligonucleotide; and (ii) a hybridization intensity value for the target oligonucleotide and the probe oligonucleotide at a probe spot. The measure of affinity value and the hybridization intensity value are used to determine the concentration level of the target nucleic acid.
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