Isometric primer extension method and kit for detection and quantification of specific nucleic acid
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United States of America Patent
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Nov 30, 2004
Grant Date -
Aug 7, 2003
app pub date -
May 23, 2001
filing date -
Jun 8, 2000
priority date (Note) -
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Abstract
A method for detecting and/or quantifying a target DNA or RNA present in a sample by a isometric primer extension method is disclosed. The method includes carrying out a primer extension reaction in the absence of a free nucleotide so that the primer extension reaction is stopped where the absent nucleotide would have been inserted. Thus, as the amount of incorporation of a labeled nucleotide on the primer extended product is detected, the amount of the target RNA or DNA in the sample is measured.
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