Direct detection of bacteria-antibody complexes via UV resonance Raman spectroscopy
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United States of America Patent
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Jan 18, 2005
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N/A
app pub date -
Mar 14, 1997
filing date -
Mar 14, 1997
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Expired
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Abstract
A system for the detection of bacteria based on bacteria-antibody complexes. Bacteria attached to antibody are detected with resonance Raman spectroscopy. The bacteria are detected directly in a great numerical excess, e.g. 100 to 10,000 of antibody molecules. A sample to be tested is placed in a medium, the medium containing antibodies attached to a surface for binding to a specific bacteria to form an antigen to antibody complex. The medium is contacted with a beam of light energy. The bacteria, as a lower resonance enhanced Raman backscattered energy, is analyzed for the presence or absence of the bacteria.
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