Molecular control of transgene segregation and its escape by a recoverable block of function (RBF) system
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United States of America Patent
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Feb 1, 2005
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Jul 14, 2000
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Jul 14, 2000
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Abstract
The invention is related to a method and a DNA construct for controlling transgene segregation in a sexually reproducing multicellular organism (SRMO), especially in such SRMOs which are susceptible of interbreeding with their cultured or wild-type relatives. The method and construct allows the farmer to reuse the transgenic crop without risk for escape of the transgene into the environment. The DNA construct is a recoverable block of function (RBF) system comprising at least one blocking construct (BC), and at least one means for recovering the blocked functions. The BC has a capacity of blocking at least one molecular or physiological function essential for the development and/or reproductive cycle of the SRMO. The BC is closely linked to the transgene of intereset (TGI). The blocked function is recoverable by an external treatment or intervention, which is optionally combined with one or more recovering constructs (RC). Different types of RBFs, including single, full, delayed, double, multiple and reversed delayed RBF are disclosed.
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