Viral amplification of recombinant messenger RNA in transgenic plants
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United States of America Patent
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Feb 8, 2005
Grant Date -
Aug 1, 2002
app pub date -
Aug 14, 2001
filing date -
Dec 30, 1992
priority date (Note) -
Expired
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Abstract
A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus, the recombinant transcripts are replicated producing high levels of foreign gene RNA.
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