Method of selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation
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United States of America Patent
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May 31, 2005
Issued Date -
N/A
app pub date -
Nov 13, 1995
filing date -
Apr 15, 1994
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernatant can be further enriched by multiple removal steps.
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