Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
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United States of America Patent
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Aug 2, 2005
Grant Date -
Sep 12, 2002
app pub date -
Feb 22, 2002
filing date -
Dec 21, 1999
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Abstract
An improved method of forming a specific complex between a probe containing probe nucleobases and a target containing target nucleobases, includes mixing the probe and the target under hybridizing conditions, wherein the probe and/or the target is conjugated to a blocking agent, which enhances the avidity and/or specificity of hybridization, whether by Watson-Crick motif or by homologous binding motif. The blocking agent contains at least one nucleobase and can be, e.g., a free nucleobase, a nucleoside or a nucleotide.
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