Ligand based solution assay for low concentration analytes
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United States of America Patent
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Aug 30, 2005
Grant Date -
Jun 17, 2004
app pub date -
Dec 1, 2003
filing date -
Oct 14, 2000
priority date (Note) -
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Abstract
The present invention provides an assay for an analyte in solution. The assay relies, in part, on associating two catalytic activities in close proximity to each other (e.g., by conjugating each enzyme to a ligand that binds the analyte) and providing substrates that produce a colored product only when both activities are bound to the same molecule in solution.
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