De novo or "universal" sequencing array
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United States of America Patent
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Sep 20, 2005
Grant Date -
Oct 10, 2002
app pub date -
Jun 29, 2001
filing date -
Nov 21, 1997
priority date (Note) -
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Abstract
The present invention describes a novel nucleic acid sequencing reagent which consists of a capture moiety, a spacer region and a sequence specific hybridizing region of 4-8 bases. The nucleic acid sequencing reagent of the present invention can also contain an attachment moiety. The nucleic acid sequencing reagent can be arranged into a nested array. This array configuration can then be used to sequence a given template without prior knowledge (de novo) of the wild type or expected sequence in conjunction with primer extension in the presence of a labeled chain terminating nucleotide.
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