Primers useful for sizing nucleic acids
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United States of America Patent
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Sep 27, 2005
Issued Date -
N/A
app pub date -
Aug 25, 1998
filing date -
May 22, 1995
priority date (Note) -
Expired
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Abstract
The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a 3′ portion of the primer (linked to an extension product) can be released from an upstream 5′ portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
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