High throughput DNA sequencing vector
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United States of America Patent
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Oct 18, 2005
Grant Date -
Jun 6, 2002
app pub date -
May 4, 2001
filing date -
Apr 10, 1998
priority date (Note) -
Expired
status (Latency Note)
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Abstract
High throughput DNA sequencing vectors for generating nested deletions using enzymatic techniques and/or transposition-based techniques are disclosed. Methods of constructing contigs of long DNA sequences and methods of generating nested deletions are also disclosed. A truncated lacZ derivative useful in measuring the copy number of the lacZ derivative in a host cell is also disclosed.
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