Circular DNA molecule with conditional origin of replication, method for preparing the same and use thereof in gene therapy
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United States of America Patent
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Dec 20, 2005
Grant Date -
Aug 16, 2001
app pub date -
Sep 13, 1996
filing date -
Sep 15, 1995
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Abstract
A circular DNA molecule, useful for gene therapy, comprising at least one nucleic acid sequence of interest, characterized in that the region allowing the replication thereof has an origin of replication with a functionality in a host cell that requires the presence of at least one specific protein foreign to said host cell. A method for preparing same, cells incorporating said DNA molecules and uses thereof in gene therapy are also described.
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