Method for extracting DNA from organisms
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United States of America Patent
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Jan 24, 2006
Grant Date -
Mar 13, 2003
app pub date -
Oct 28, 2002
filing date -
Apr 26, 2000
priority date (Note) -
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Abstract
A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and the DNA subjected to a first alternating field electrophoresis to extract DNA fragments which are large in size and separate them from the cell debris. The first electrophoretic migration is followed by an enzymatic restriction step and additional electrophoretic migrations. The invention also encompasses DNA obtained by the method.
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