Methods and compositions for use in perfusion applications
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United States of America Patent
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Jan 31, 2006
Grant Date -
Jan 29, 2004
app pub date -
May 23, 2003
filing date -
Feb 3, 1999
priority date (Note) -
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status (Latency Note)
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Abstract
Methods and compositions for use in perfusion applications are provided. In the subject methods, a subject or derivative thereof, e.g. isolated organ or tissue, is infused, perfused and/or transfused with at least two fluid compositions. The first fluid composition is a non-naturally occurring biological buffer free plasma-like solution and the second fluid composition is a fluid blood composition. In a preferred embodiment, an additional volume of the first solution, or a derivative thereof, is administered to the patient following introduction of the fluid blood composition. Also provided are kits and systems for performing the subject methods. The subject methods and compositions find use in variety of perfusion applications, including the treatment of hypothermic surgical applications, cryogenic procedures, and the like.
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