Plasmids for plant transformation and method for using the same
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United States of America Patent
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Apr 18, 2006
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N/A
app pub date -
Dec 22, 1999
filing date -
Jun 30, 1997
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Expired
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Abstract
The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
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