Method for detecting cockroach allergens and determining total allergen level
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United States of America Patent
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Apr 25, 2006
Grant Date -
Sep 25, 2003
app pub date -
Mar 19, 2003
filing date -
Mar 22, 2002
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention is directed to a method and a test kit for detecting cockroach allergens and/or determining the total allergen level. A sample from an environment in which cockroaches are present or are suspected to be present is provided. The sample and a substrate composition comprising a chitinase substrate are then contacted. The presence of cockroach allergens can be determined by observing or detecting the magnitude of a measurable change of a property of the substrate composition following the contact between the sample and the substrate composition wherein the magnitude of the measurable change of a property of the substrate composition is proportional to the total allergen level. The total allergen level can be determined by measuring the magnitude of the measurable change of a property of the substrate composition, or by comparing the magnitude with a reference standard.
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