Recombinant polyketide synthase genes
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United States of America Patent
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Apr 25, 2006
Grant Date -
Nov 21, 2002
app pub date -
Apr 18, 2002
filing date -
Oct 8, 1999
priority date (Note) -
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Abstract
Regions of sequence identity are recurrent in some modular polyketide synthase (PKS) gene clusters. Such sequences are potentially detrimental to the stability of PKS gene clusters and expression plasmids for the genes in the gene cluster. PKS gene and gene cluster stability can be improved, and reproducible polyketide titers can be obtained using those genes and gene clusters when the regions of sequence identity are reduced or eliminated by replacing one or more identical or homologous segments with non-homologous segments that encode the same or a substantially similar amino acid sequence.
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