Method for manufacturing a tissue factor-based prothrombin time reagent
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United States of America Patent
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May 23, 2006
Grant Date -
May 6, 2004
app pub date -
Nov 5, 2002
filing date -
Nov 5, 2002
priority date (Note) -
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Abstract
A method for manufacturing a tissue factor-based prothrombin-time reagent includes combining predetermined quantities of tissue factor (TF), phospholipid and detergent-containing buffer to create a TF/phospholipid mixture. In this combining step, the quantity of TF is predetermined based upon its measured TF activity. Next, the detergent is removed from the TF/phospholipid mixture to produce an essentially detergent-free TF/phospholipid mixture that is useful as a tissue factor-based PT reagent. The method can include the use of hydrophobic resin that has been prescreened based on phospholipid recovery to accomplish the removal of the detergent.
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