Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
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United States of America Patent
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May 23, 2006
Grant Date -
Dec 19, 2002
app pub date -
Mar 22, 2002
filing date -
Dec 18, 1996
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Abstract
A multiple-plasmid system for heterologous expression of polyketides facilitates combinatorial biosynthesis. The method can be extended to any modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) and has the potential to produce thousands of novel natural products, including ones derived from further modification of the PKS or NRPS products by tailoring enzymes.
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