Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
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United States of America Patent
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Jun 6, 2006
Grant Date -
Mar 17, 2005
app pub date -
Aug 20, 2002
filing date -
Aug 20, 2001
priority date (Note) -
Expired
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Abstract
Methods of amplifying a target nucleic acid whereby the target nucleic acid is amplified in the presence of a deoxyribonucleotide triphosphate, a DNA polymerase having strand displacement activity, at least one chimeric oligonucleotide primer, at least one upstream block oligonucleotide and a RNase H; wherein the chimeric oligonucleotide primer contains a ribonucleotide positioned at the 3'-terminus; wherein the upstream block oligonucleotide is capable of annealing to a region 3' to a portion in the nucleic acid as the template to which the chimeric oligonucleotide primer anneals; and compositions and kits thereof.
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