Method for the recovery and purification of poxviruses from infected cells
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United States of America Patent
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Jun 6, 2006
Grant Date -
Dec 30, 2004
app pub date -
Dec 13, 2002
filing date -
Dec 20, 2001
priority date (Note) -
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Abstract
The present invention relates to a method for the recovery of poxviruses, in particular modified Vaccinia virus Ankara (MVA), from infected cells. According to the present invention the virus-infected cells are subjected to a high-pressure homogenization to obtain a virus containing homogenate. The virus containing homogenate can be subjected to at least one purification step to obtain apoxvirus-enriched fraction. The invention further relates to the virus containing fraction and the virus containing homogenate obtained by the method according to the present invention.
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