Dewaxing kit for immunostaining tissue specimens
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United States of America Patent
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Jul 4, 2006
Grant Date -
Jul 1, 2004
app pub date -
Aug 29, 2003
filing date -
Mar 11, 1994
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
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Family
- 15 United States
- 10 France
- 8 Japan
- 7 China
- 5 Korea
- 2 Other
Patent Owner(s)
- BIOGENEX LABORATORIES
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- Non-US Classification not provided for expired patents
Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Kalra, Krishan L | Danville, CA | 23 | 1577 |
Su, Sheng-Hui | San Ramon, CA | 9 | 484 |
Yu, Cheng-Zhi | Pleasant Hill, CA | 6 | 284 |
Zhang, Guangrong | San Ramon, CA | 9 | 222 |
Zhou, Ding | Pleasant Hill, CA | 62 | 1541 |
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Maintenance Fees
Fee | Large entity fee | small entity fee | micro entity fee | due date |
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Fee | Large entity fee | small entity fee | micro entity fee |
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Surcharge after expiration - Late payment is unavoidable | $700.00 | $350.00 | $175.00 |
Surcharge after expiration - Late payment is unintentional | $1,640.00 | $820.00 | $410.00 |
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