Cassette, system, and 2-D gel electrophoresis method for separating molecules
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United States of America Patent
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Dec 26, 2006
Grant Date -
Mar 11, 2004
app pub date -
Apr 9, 2003
filing date -
Apr 12, 2002
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A gel electrophoresis cassette (33) is disclosed which includes two plates (34, 35) and at least one seal (36) which separates these plates. The seal (36) is annular; it may be positioned essentially in the region of the outer edge of the plates (34, 35). For performing an electrophoresis in a second dimension following an isoelectric focusing in an first dimension, one of the plates (34, 35) includes a recess (37) for inserting a strip holder (1) having a base plate (4), which has a carrier surface (2) on which an IEF strip (3) is accommodated. The base plate (4) includes at least one stop (5) offset to a lower level in relation to the carrier surface (2) and a sealing surface (6). The strip holder (1) is insertable into this recess (37) in such a way that the stop (5) of the base plate (4) is applied to the outer surface of the plate (34, 35) and the sealing surface (6) presses tightly against the inner surface (38) of the recess (37). In addition, systems and methods for performing a 2-D gel electrophoresis are disclosed.
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